Genetic analysis of the Sinorhizobium meliloti BacA protein: differential effects of mutations on phenotypes.

ABSTRACT: Sinorhizobium meliloti strains lacking BacA function are impaired in symbiosis with alfalfa host plants and display altered sensitivities to a number of compounds relative to wild-type strains. With the goal of finding clues to the currently unknown biological function(s) of BacA, we carried out a genetic analysis to determine which amino acids are critical for protein function and to attempt to ascertain whether the multiple phenotypes that result from a bacA-null allele were the result of a common cause or whether BacA has multiple functions. We have created a set of 20 site-directed mutants in which selected individual amino acids in bacA were replaced with glycine residues. The resulting mutants were characterized to determine how the various amino acid changes affected a number of phe

SUBMITTER: LeVier K 

PROVIDER: S-EPMC100141 | biostudies-literature | 2001 Nov

REPOSITORIES: biostudies-literature