Detection of chromosomal alteration after infusion of gene-edited allogeneic CAR T cells.

ABSTRACT: A chromosome 14 inversion was found in a patient who developed bone marrow aplasia following treatment with allogeneic chimeric antigen receptor (CAR) Tcells containing gene edits made with transcription activator-like effector nucleases (TALEN). TALEN editing sites were not involved at either breakpoint. Recombination signal sequences (RSSs) were found suggesting recombination-activating gene (RAG)-mediated activity. The inversion represented a dominant clone detected in the context of decreasing absolute CAR Tcell and overall lymphocyte counts. The inversion was not associated with clinical consequences and wasnot detected in the drug product administered to this patient or in any drug product used in this or other trials using the same manufacturing processes. Neither was the inversion

SUBMITTER: Sasu BJ 

PROVIDER: S-EPMC10014221 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature