Ginsenoside Rb2 suppresses cellular senescence of human dermal fibroblasts by inducing autophagy.

ABSTRACT:

Background

Ginsenoside Rb2, a major active component of Panax ginseng, has various physiological activities, including anticancer and anti-inflammatory effects. However, the mechanisms underlying the rejuvenation effect of Rb2 in human skin cells have not been elucidated.

Methods

We performed a senescence-associated β-galactosidase staining assay to confirm cellular senescence in human dermal fibroblasts (HDFs). The regulatory effects of Rb2 on autophagy were evaluated by analyzing the expression of autophagy marker proteins, such as microtubule-associated protein 1A/1B-light chain (LC) 3 and p62, using immunoblotting. Autophagosome and autolysosome formation was monitored using transmission electron microscopy. Autophagic flux was analyzed using tandem-labeled GFP-RF

SUBMITTER: Yang KE 

PROVIDER: S-EPMC10014224 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature