Bioactive Fibronectin-III₁₀-DNA Origami Nanofibers Promote Cell Adhesion and Spreading.

ABSTRACT: The integration of proteins with DNA nanotechnology would enable materials with diverse applications in biology, medicine, and engineering. Here, we describe a method for the incorporation of bioactive fibronectin domain proteins with DNA nanostructures using two orthogonal coiled-coil peptides. One peptide from each coiled-coil pair is attached to a DNA origami cuboid in a multivalent fashion by attaching the peptides to DNA handles. These structures can then be assembled into one-dimensional arrays through the addition of a fibronectin domain linker genetically fused with the complementary peptides to those on the origami. We validate array formation using two different self-assembly protocols and characterize the fibers by atomic force and electron microscopy. Finally, we demonstrate th

SUBMITTER: Buchberger A 

PROVIDER: S-EPMC10014493 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature