Tissue factor activity of small and large extracellular vesicles in different diseases.

ABSTRACT:

Background

Circulating procoagulant extracellular vesicles (EVs) are increased in diseases, such as cancer, sepsis, and COVID-19. EV tissue factor (TF) activity is associated with disseminated intravascular coagulation in sepsis and venous thrombosis in patients with pancreatic cancer and COVID-19. EVs are commonly isolated by centrifugation at ∼20,000 g.

Objectives

In this study, we analyzed the TF activity of 2 EV populations enriched for large and small EVs in patients with either sepsis, pancreatic cancer, or COVID-19.

Methods

EVs were isolated from plasma by sequential centrifugation at 20,000 g (large EVs, LEVs) and then 100,000 g (small EVs, SEVs). We analyzed EVs from plasma prepared from whole blood samples from healthy individuals with or without lipopolysac

SUBMITTER: Sachetto ATA 

PROVIDER: S-EPMC10015082 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature