Simple Technique for Microscopic Evaluation of Active Cellular Invasion into 3D Hydrogel Constructs.

ABSTRACT: Materials that are evaluated for bioengineering purposes are carefully tested to evaluate cellular interactions with respect to biocompatibility and in some cases cell differentiation. A key perspective that is often considered is the ability for decellularized synthetic or natural based matrices to facilitate cell migration or tissue ingrowth. Current methods of measuring cell migration range from simple scratch assays to Boyden chamber inserts and fluorescent imaging of seeded spheroids. Many of these methods require tissue processing for histological analysis and fixing and staining for imaging, which can be difficult and dependent on the stability of the hydrogel subject. Herein we present a simple platform that can be manufactured using 3D printing and easily applied to in vitro cell

SUBMITTER: Simpson CR 

PROVIDER: S-EPMC10015425 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature