Coexistence of tmexCD3-toprJ1b tigecycline resistance genes with two novel bla _VIM-2-carrying and bla _OXA-10-carrying transposons in a Pseudomononas asiatica plasmid.

ABSTRACT:

Introduction

Tigecycline and carbapenems are considered the last line of defense against microbial infections. The co-occurrence of resistance genes conferring resistance to both tigecycline and carbapenems in Pseudomononas asiatica was not investigated.

Methods

P. asiatica A28 was isolated from hospital sewage. Antibiotic susceptibility testing showed resistance to carbapenem and tigecycline. WGS was performed to analyze the antimicrobial resistance genes and genetic characteristics. Plasmid transfer by conjugation was investigated. Plasmid fitness costs were evaluated in Pseudomonas aeruginosa transconjugants including a Galleria mellonella infection model.

Results

Meropenem and tigecycline resistant P. asiatica A28 carries a 199,

SUBMITTER: Li Q 

PROVIDER: S-EPMC10015498 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature