Molecular detection and genotyping of Human Cytomegalovirus in kidney transplant recipients under ganciclovir prophylaxis in Iran.

ABSTRACT:

Introduction

Human Cytomegalovirus (HCMV) infection is one of the most common viral complications in kidney transplant recipients. Although there are effective treatments strategies for the HCMV infection, this infection is still one of the causes of kidney transplant rejection.

Methods

A total of 246 kidney transplant recipients participated in this cross-sectional study. Viral DNA was extracted from these plasma samples and the presence of HCMV genome was determined by Semi-nested PCR with specific primers for HCMV B glycoprotein gene. Sanger sequencing analyses were carried out to determine HCMV genotypes and Mega x software was used for nucleotide alignment and construction of a phylogenetic tree.

Results

Human cytomegalovirus DNA was detected in 11 (4.47%) recipi

SUBMITTER: Behnezhad F 

PROVIDER: S-EPMC10015764 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature