In Vitro Selection Identifies Staphylococcus aureus Genes Influencing Biofilm Formation.

ABSTRACT: Staphylococcus aureus generates biofilms during many chronic human infections, which contributes to its growth and persistence in the host. Multiple genes and pathways necessary for S. aureus biofilm production have been identified, but knowledge is incomplete, and little is known about spontaneous mutations that increase biofilm formation as infection progresses. Here, we performed in vitro selection of four S. aureus laboratory strains (ATCC 29213, JE2, N315, and Newman) to identify mutations associated with enhanced biofilm production. Biofilm formation increased in passaged isolates from all strains, exhibiting from 1.2- to 5-fold the capacity of parental lines. Whole-genome sequencing identified nonsynonymous mutations affecting 23 candidate genes and a genomic duplication enco

SUBMITTER: Long DR 

PROVIDER: S-EPMC10016075 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature