Sense and antisense RNA products of the uxuR gene can affect motility and chemotaxis acting independent of the UxuR protein.

ABSTRACT: Small non-coding and antisense RNAs are widespread in all kingdoms of life, however, the diversity of their functions in bacteria is largely unknown. Here, we study RNAs synthesised from divergent promoters located in the 3'-end of the uxuR gene, encoding transcription factor regulating hexuronate metabolism in Escherichia coli. These overlapping promoters were predicted in silico with rather high scores, effectively bound RNA polymerase in vitro and in vivo and were capable of initiating transcription in sense and antisense directions. The genome-wide correlation between in silico promoter scores and RNA polymerase binding in vitro and in vivo was higher for promoters located on the antisense strands of the genes, however, sense prom

SUBMITTER: Tutukina MN 

PROVIDER: S-EPMC10016265 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature