In-Depth Proteomic Analysis of De Novo Proteome in a Mouse Model of Alzheimer's Disease.

ABSTRACT:

Background

Alzheimer's disease (AD) is the most common dementia syndrome in the elderly characterized by synaptic failure and unique brain pathology. De novo protein synthesis is required for the maintenance of memory and synaptic plasticity. Mounting evidence links impaired neuronal protein synthesis capacity and overall protein synthesis deficits to AD pathogenesis. Meanwhile, identities of AD-associated dysregulation of "newly synthesized proteome" remain elusive.

Objective

To investigate de novo proteome alterations in the hippocampus of aged Tg19959 AD model mice.

Methods

In this study, we combined the bioorthogonal noncanonical amino acid tagging (BONCAT) method with the unbiased large-scale proteomic analysis in acute living brain slices (we name it "BONSPEC")

SUBMITTER: Wang X 

PROVIDER: S-EPMC10016629 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature