Development and Characterization of Nanobody-Derived CD47 Theranostic Pairs in Solid Tumors.

ABSTRACT: Overexpression of CD47 is frequently observed in various types of human malignancies, inhibiting myeloid-mediated elimination of tumor cells and affecting the prognosis of cancer patients. By mapping biomarker expression, immuno-positron emission tomography has been increasingly used for patient screening and response monitoring. By immunization alpacas with recombinant human CD47, we prepared a CD47-targeting nanobody C2 and developed [⁶⁸Ga]Ga-NOTA-C2, followed by an exploration of the diagnostic value in CD47-expressing tumor models including gastric-cancer patient-derived xenograft models. By fusing C2 to an albumin binding domain (ABD), we synthesized ABDC2, which had increased in vivo half-life and improved targeting properties. We further labeled ABDC2 with ⁶⁸Ga

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10017100 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature