Mitochondrial stress and glycoxidation increase with decreased kidney function.

ABSTRACT: Mitochondrial stress increases the production of fumarate, an intermediate of the Krebs cycle. Fumarate non-enzymatically reacts with the thiol group of cysteine, leading to the production of S-(2-succinyl)cysteine. Here, we quantified the concentration of fumarate, the free form of S-(2-succinyl)cysteine, and advanced glycation end-products, including N ^ε-(carboxymethyl)lysine and N ^δ-(5-hydro-5-methyl-4-imidazolone-2-yl)-ornithine, in the serum of chronic kidney disease patients, using liquid chromatography-tandem mass spectrometry and an enzymatic assay. In a cross-sectional study, we evaluated the difference in metabolite concentration between healthy individuals (n = 22) and kidney transplant patients (n = 93). Additional

SUBMITTER: Katsuta N 

PROVIDER: S-EPMC10017327 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature