Spheromers reveal robust T cell responses to the Pfizer/BioNTech vaccine and attenuated peripheral CD8⁺ T cell responses post SARS-CoV-2 infection.

ABSTRACT: T cells are a critical component of the response to SARS-CoV-2, but their kinetics after infection and vaccination are insufficiently understood. Using "spheromer" peptide-MHC multimer reagents, we analyzed healthy subjects receiving two doses of the Pfizer/BioNTech BNT162b2 vaccine. Vaccination resulted in robust spike-specific T cell responses for the dominant CD4⁺ (HLA-DRB1^∗15:01/S191) and CD8⁺ (HLA-A^∗02/S691) T cell epitopes. Antigen-specific CD4⁺ and CD8⁺ T cell responses were asynchronous, with the peak CD4⁺ T cell responses occurring 1 week post the second vaccination (boost), whereas CD8⁺ T cells peaked 2 weeks later. These peripheral T cell responses were elevated compared with COVID-19 patient

SUBMITTER: Gao F 

PROVIDER: S-EPMC10017386 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature