Muscle progenitor cells are required for skeletal muscle regeneration and prevention of adipogenesis after limb ischemia.

ABSTRACT: Skeletal muscle injury in peripheral artery disease (PAD) has been attributed to vascular insufficiency, however evidence has demonstrated that muscle cell responses play a role in determining outcomes in limb ischemia. Here, we demonstrate that genetic ablation of Pax7⁺ muscle progenitor cells (MPCs) in a model of hindlimb ischemia (HLI) inhibited muscle regeneration following ischemic injury, despite a lack of morphological or physiological changes in resting muscle. Compared to control mice (Pax7^WT), the ischemic limb of Pax7-deficient mice (Pax7^Δ) was unable to generate significant force 7 or 28 days after HLI. A significant increase in adipose was observed in the ischemic limb 28 days after HLI in Pax7^Δ mice, which replaced functional muscle

SUBMITTER: Abbas H 

PROVIDER: S-EPMC10017542 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature