Simple, fast and inexpensive quantification of glycolate in the urine of patients with primary hyperoxaluria type 1.

ABSTRACT: In primary hyperoxaluria type 1 excessive endogenous production of oxalate and glycolate leads to increased urinary excretion of these metabolites. Although genetic testing is the most definitive and preferred diagnostic method, quantification of these metabolites is important for the diagnosis and evaluation of potential therapeutic interventions. Current metabolite quantification methods use laborious, technically highly complex and expensive liquid, gas or ion chromatography tandem mass spectrometry, which are available only in selected laboratories worldwide. Incubation of ortho-aminobenzaldehyde (oABA) with glyoxylate generated from glycolate using recombinant mouse glycolate oxidase (GO) and glycine leads to the formation of a stable dihydroquinazoline double aromatic ring chromophor

SUBMITTER: Boehm T 

PROVIDER: S-EPMC10017573 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature