DNA methyltransferase inhibition induces dynamic gene expression changes in lung CD4⁺ T cells of neonatal mice with E. coli pneumonia.

ABSTRACT: Bacterial pulmonary infections are a major cause of morbidity and mortality in neonates, with less severity in older children. Previous studies demonstrated that the DNA of CD4⁺ T cells in the mouse lung, whose primary responsibility is to coordinate the immune response to foreign pathogens, is differentially methylated in neonates compared with juveniles. Nevertheless, the effect of this differential DNA methylation on CD4⁺ T cell gene expression and response to infection remains unclear. Here we treated E. coli-infected neonatal (4-day-old) and juvenile (13-day-old) mice with decitabine (DAC), a DNA methyltransferase inhibitor with broad-spectrum DNA demethylating activity, and performed simultaneous genome-wide DNA methylation and transcriptional profiling on lung

SUBMITTER: Michki SN 

PROVIDER: S-EPMC10017701 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature