Pyrophosphate inhibits periodontal ligament stem cell differentiation and mineralization through MAPK signaling pathways.

ABSTRACT:

Background and objective

Periodontal ligament stem cells (PDLSCs) are the primary cell source for the regeneration and remodeling of periodontal ligament (PDL). It is crucial to prevent PDLSCs from mineralization when using the PDLSCs for PDL regeneration. At present, little is known about how to inhibit PDLSC mineralization. This study investigates the effects of pyrophosphate (PPi) on inhibiting PDLSC osteogenic differentiation and mineralization as well as the underlying mechanism.

Materials and methods

Human PDLSCs were cultured in an osteogenic differentiation medium with different PPi concentrations (0, 10, or 100 μM). The effects of PPi on osteogenic differentiation were assessed by ALP activity and the expressions of osteogenic related proteins (OPN, RUNX2, OSX, and

SUBMITTER: Liang Y 

PROVIDER: S-EPMC10018283 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature