PTBP1 controls intestinal epithelial regeneration through post-transcriptional regulation of gene expression.

ABSTRACT: The intestinal epithelial regeneration is driven by intestinal stem cells under homeostatic conditions. Differentiated intestinal epithelial cells, such as Paneth cells, are capable of acquiring multipotency and contributing to regeneration upon the loss of intestinal stem cells. Paneth cells also support intestinal stem cell survival and regeneration. We report here that depletion of an RNA-binding protein named polypyrimidine tract binding protein 1 (PTBP1) in mouse intestinal epithelial cells causes intestinal stem cell death and epithelial regeneration failure. Mechanistically, we show that PTBP1 inhibits neuronal-like splicing programs in intestinal crypt cells, which is critical for maintaining intestinal stem cell stemness. This function is achieved at least in part through promotin

SUBMITTER: Chembazhi UV 

PROVIDER: S-EPMC10018364 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature