Reconstruction and deconstruction of human somitogenesis in vitro.

ABSTRACT: The vertebrate body displays a segmental organization that is most conspicuous in the periodic organization of the vertebral column and peripheral nerves. This metameric organization is first implemented when somites, which contain the precursors of skeletal muscles and vertebrae, are rhythmically generated from the presomitic mesoderm. Somites then become subdivided into anterior and posterior compartments that are essential for vertebral formation and segmental patterning of the peripheral nervous system^1-4. How this key somitic subdivision is established remains poorly understood. Here we introduce three-dimensional culture systems of human pluripotent stem cells called somitoids and segmentoids, which recapitulate the formation of somite-like structures with anteroposterior

SUBMITTER: Miao Y 

PROVIDER: S-EPMC10018515 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature