A pangenome approach-based loop-mediated isothermal amplification assay for the specific and early detection of Bordetella pertussis.

ABSTRACT: Despite widespread vaccination, Bordetella pertussis continues to cause pertussis infections worldwide, leaving infants at the highest risk of severe illness and death, while people around them are likely the main sources of infection and rapidly spread the disease. Rapid and less complex molecular testing for the specific and timely diagnosis of pertussis remains a challenge that could help to prevent the disease from worsening and prevent its transmission. We aimed to develop and validate a colorimetric loop-mediated isothermal amplification (LAMP) assay using a new target uvrD_2 informed by the pangenome for the specific and early detection of B. pertussis. Compared to that of multitarget quantitative polymerase chain reaction (multitarget qPCR) using a large clinical DNA specimen (n = 

SUBMITTER: Juscamayta-Lopez E 

PROVIDER: S-EPMC10018623 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature