C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining.

ABSTRACT: The C. elegans germline makes an excellent model for studying meiosis, in part due to the ease of conducting cytological analyses on dissected animals. Whole mount preparations preserve the structure of meiotic nuclei, and importantly, each gonad arm contains all stages of meiosis, organized in a temporal-spatial progression that makes it easy to identify nuclei at different stages. Adult hermaphrodites have two gonad arms, each organized as a closed tube with proliferating germline stem cells at the distal closed end and cellularized oocytes at the proximal open end, which join in the center at the uterus. Dissection releases one or both gonad arms from the body cavity, allowing the entirety of meiosis to be visualized. Here, a common protocol for immunofluorescence against a protein of i

SUBMITTER: Ananthaswamy D 

PROVIDER: S-EPMC10018647 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature