Effects of neuronal cell adhesion molecule L1 and nanoparticle surface modification on microglia.

ABSTRACT: Microelectrode arrays for neural recording suffer from low yield and stability partly due to the inflammatory host responses. A neuronal cell adhesion molecule L1 coating has been shown to promote electrode-neuron integration, reduce microglia activation and improve recording. Coupling L1 to surface via a nanoparticle (NP) base layer further increased the protein surface density and stability. However, the exact L1-microglia interaction in these coatings has not been studied. Here we cultured primary microglia on L1 modified surfaces (with and without NP) and characterized microglia activation upon phorbol myristate acetate (PMA) and lipopolysaccharide (LPS) stimulation. Results showed L1 coatings reduced microglia's superoxide production in response to PMA and presented intrinsic antioxid

SUBMITTER: Kushwah N 

PROVIDER: S-EPMC10018678 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature