Exogenous mitochondrial transplantation improves survival and neurological outcomes after resuscitation from cardiac arrest.

ABSTRACT:

Background

Mitochondrial transplantation (MTx) is an emerging but poorly understood technology with the potential to mitigate severe ischemia-reperfusion injuries after cardiac arrest (CA). To address critical gaps in the current knowledge, we test the hypothesis that MTx can improve outcomes after CA resuscitation.

Methods

This study consists of both in vitro and in vivo studies. We initially examined the migration of exogenous mitochondria into primary neural cell culture in vitro. Exogenous mitochondria extracted from the brain and muscle tissues of donor rats and endogenous mitochondria in the neural cells were separately labeled before co-culture. After a period of 24 h following co-culture, mitochondrial transfer was observed using microscopy. In vitro adenosine tripho

SUBMITTER: Hayashida K 

PROVIDER: S-EPMC10018842 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature