Relationship between tumor microbiota transcriptional activity and gene expression in breast cancer.

ABSTRACT:

Background

A few studies have reported the distribution of the microbiota in breast cancer tissues, but few reports have compared the microbiota in different subtypes of breast cancer tissue. Moreover, no study has reported on the relationship between the microbiota and gene expression in breast tumor.

Methods

Sections of formalin-fixed paraffin-embedded (FFPE) tissue were prepared from the breast tumors of 70 patients and were subjected to microarray analysis to identify gene expression profiles. The same total RNA samples were also used to analyze the microbiota activity in tumor tissues by performing 16 S rRNA sequencing and internal transcribed spacer (ITS) sequencing of reverse transcript cDNA with Illumina Miseq. Pearson's correlation coefficient was used for calculati

SUBMITTER: Liu E 

PROVIDER: S-EPMC10018882 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature