Exposure to oxLDL impairs TGF-β activity in human tendon cells.

ABSTRACT:

Background

Previous studies have shown that patients with hypercholesterolemia experience elevated levels of oxidized LDL (oxLDL), a molecule which triggers inflammation and collagenase activity. In this study we discovered novel mechanistic effects of oxLDL on tendon cells and the mediators regulating matrix remodeling by analyzing the expression and activity of related proteins and enzymes. These effects may contribute to tendon damage in patients with high cholesterol.

Methods

Isolated human tendon cells (male and female donors age 28 ± 1.4 age 37 ± 5.7, respectively) were incubated in the presence or absence of oxLDL. The influence of oxLDL on the expression level of key mRNA and proteins was examined using real time quantitative PCR, ELISA and Western blots. The activit

SUBMITTER: Mousavizadeh R 

PROVIDER: S-EPMC10018928 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature