Spectrally Resolved Fiber Photometry for In Vivo Multi-Color Fluorescence Measurements.

ABSTRACT: This article describes how to assemble and operate a spectrometer-based fiber photometry system for in vivo simultaneous measurements of multiple fluorescent biosensors in freely moving mice. The first section of the article describes the step-by-step procedure to assemble a basic single-spectrometer fiber photometry system and how to expand it into a dual-spectrometer system that allows for simultaneous recordings from two sites. The second part describes the steps for a typical fiber probe implantation surgery. The last section describes how to acquire and analyze the time-lapsed spectral data. This article is intended for teaching labs how to build their own fiber photometry systems (with a video tutorial) from commercially available parts and perform in vivo recordings in behaving mice

SUBMITTER: Zhou J 

PROVIDER: S-EPMC10018997 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature