Understanding repertoire sequencing data through a multiscale computational model of the germinal center.

ABSTRACT: Sequencing of B-cell and T-cell immune receptor repertoires helps us to understand the adaptive immune response, although it only provides information about the clonotypes (lineages) and their frequencies and not about, for example, their affinity or antigen (Ag) specificity. To further characterize the identified clones, usually with special attention to the particularly abundant ones (dominant), additional time-consuming or expensive experiments are generally required. Here, we present an extension of a multiscale model of the germinal center (GC) that we previously developed to gain more insight in B-cell repertoires. We compare the extent that these simulated repertoires deviate from experimental repertoires established from single GCs, blood, or tissue. Our simulations show that there

SUBMITTER: Garcia-Valiente R 

PROVIDER: S-EPMC10019394 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature