A novel approach to measure complex V ATP hydrolysis in frozen cell lysates and tissue homogenates.

ABSTRACT: Mitochondrial depolarization can initiate reversal activity of ATP synthase, depleting ATP by its hydrolysis. We have recently shown that increased ATP hydrolysis contributes to ATP depletion leading to a maladaptation in mitochondrial disorders, where maximal hydrolytic capacity per CV content is increasing. However, despite its importance, ATP hydrolysis is not a commonly studied parameter because of the limitations of the currently available methods. Methods that measure CV hydrolytic activity indirectly require the isolation of mitochondria and involve the introduction of detergents, preventing their utilization in clinical studies or any high-throughput analyses. Here, we describe a novel approach to assess maximal ATP hydrolytic capacity and maximal respiratory capacity in a single a

SUBMITTER: Fernandez-Del-Rio L 

PROVIDER: S-EPMC10019470 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature