HAP1, a new revolutionary cell model for gene editing using CRISPR-Cas9.

ABSTRACT: The use of next-generation sequencing (NGS) technologies has been instrumental in the characterization of the mutational landscape of complex human diseases like cancer. But despite the enormous rise in the identification of disease candidate genetic variants, their functionality is yet to be fully elucidated in order to have a clear implication in patient care. Haploid human cell models have become the tool of choice for functional gene studies, since they only contain one copy of the genome and can therefore show the unmasked phenotype of genetic variants. Over the past few years, the human near-haploid cell line HAP1 has widely been consolidated as one of the favorite cell line models for functional genetic studies. Its rapid turnover coupled with the fact that only one allele needs to

SUBMITTER: Llargues-Sistac G 

PROVIDER: S-EPMC10020200 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature