Towards standardizing basophil identification by flow cytometry

ABSTRACT:

Background

Basophils normally make up <2% of the white blood cells (WBC). There is no clear consensus for basophil identification by flow cytometry. The increased demand for basophil activation test (BAT) to identifying and monitoring allergic patients highlights the need for a standardized approach to identify basophils.

Methods

Using flow cytometry we analyzed whole blood stained with antibodies against: IgE, CD123, CD193, CD203c, CD3, HLADR, FcɛRI, CRTH2 and CD45. We examined unstimulated blood as well as blood stimulated with Anti-IgE and fMLP. Finally, we compared the results to a complete blood count (CBC) from an FDA approved hematological analyzer.

Results

Basophil identification relying on just one surface marker performed worse than approaches utilizing

SUBMITTER:  

PROVIDER: S-EPMC10020589 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature