Characterization of a novel potency endpoint for the evaluation of immune checkpoint blockade in humanized mice

ABSTRACT:

Introduction

Humanized mice are emerging as valuable models to experimentally evaluate the impact of different immunotherapeutics on the human immune system. These immunodeficient mice are engrafted with human cells or tissues, that then mimic the human immune system, offering an alternative and potentially more predictive preclinical model. Immunodeficient NSG mice engrafted with human CD34+ cord blood stem cells develop human T cells educated against murine MHC. However, autoimmune graft versus host disease (GvHD), mediated by T cells, typically develops 1 year post engraftment.

Methods

Here, we have used the development of GvHD in NSG mice, using donors with HLA alleles predisposed to autoimmunity (psoriasis) to weight in favor of GvHD, as an endpoint to evaluate the r

SUBMITTER: Matas-Cespedes A 

PROVIDER: S-EPMC10020612 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature