Oxidation modulates LINGO2-induced inactivation of large conductance, Ca²⁺-activated potassium channels.

ABSTRACT: Ca²⁺ and voltage-activated K⁺ (BK) channels are ubiquitous ion channels that can be modulated by accessory proteins, including β, γ, and LINGO1 BK subunits. In this study, we utilized a combination of site-directed mutagenesis, patch clamp electrophysiology, and molecular modeling to investigate if the biophysical properties of BK currents were affected by coexpression of LINGO2 and to examine how they are regulated by oxidation. We demonstrate that LINGO2 is a regulator of BK channels, since its coexpression with BK channels yields rapid inactivating currents, the activation of which is shifted ∼-30 mV compared to that of BKα currents. Furthermore, we show the oxidation of BK:LINGO2 currents (by exposure to epifluorescence illumination or chloramine-T) abolished inac

SUBMITTER: Dudem S 

PROVIDER: S-EPMC10020666 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature