Biosensor for Multimodal Characterization of an Essential ABC Transporter for Next-Generation Antibiotic Research.

ABSTRACT: As the threat of antibiotic resistance increases, there is a particular focus on developing antimicrobials against pathogenic bacteria whose multidrug resistance is especially entrenched and concerning. One such target for novel antimicrobials is the ATP-binding cassette (ABC) transporter MsbA that is present in the plasma membrane of Gram-negative pathogenic bacteria where it is fundamental to the survival of these bacteria. Supported lipid bilayers (SLBs) are useful in monitoring membrane protein structure and function since they can be integrated with a variety of optical, biochemical, and electrochemical techniques. Here, we form SLBs containing Escherichia coli MsbA and use atomic force microscopy (AFM) and structured illumination microscopy (SIM) as high-resolution microscopy

SUBMITTER: Bali K 

PROVIDER: S-EPMC10020959 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature