Real-Time Pyruvate Chemical Conversion Monitoring Enabled by PHIP.

ABSTRACT: In recent years, parahydrogen-induced polarization side arm hydrogenation (PHIP-SAH) has been applied to hyperpolarize [1-¹³C]pyruvate and map its metabolic conversion to [1-¹³C]lactate in cancer cells. Developing on our recent MINERVA pulse sequence protocol, in which we have achieved 27% [1-¹³C]pyruvate carbon polarization, we demonstrate the hyperpolarization of [1,2-¹³C]pyruvate (∼7% polarization on each ¹³C spin) via PHIP-SAH. By altering a single parameter in the pulse sequence, MINERVA enables the signal enhancement of C1 and/or C2 in [1,2-¹³C]pyruvate with the opposite phase, which allows for the simultaneous monitoring of different chemical reactions with enhanced spectral contrast or for the same reaction

SUBMITTER: Stevanato G 

PROVIDER: S-EPMC10021011 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature