An SPRI beads-based DNA purification strategy for flexibility and cost-effectiveness.

ABSTRACT:

Background

Current solid-phase reversible immobilization (SPRI) beads technology is widely used in molecular biology due to its convenience for DNA manipulation. However, the high performance commercial SPRI beads have no price advantage over our method. Furthermore, the use of commercially available SPRI beads standards does not provide the flexibility required for a number of specific nucleic acid handling scenarios.

Results

We report an efficient DNA purification strategy by combining home-made beads-suspension buffer with SPRI beads. The method tests the critical concentrations of polyethylene glycol (PEG) 8000 and beads to maximise recovery. And the composition of the SPRI beads DNA purification system (SDPS) was determined at 20% PEG 8000, 2 M NaCl and 16.3 mM MgCl

SUBMITTER: Liu D 

PROVIDER: S-EPMC10022144 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature