IRGD-modified memory-like NK cells exhibit potent responses to hepatocellular carcinoma.

ABSTRACT:

Background

Cytokine-induced memory-like natural killer (CIML NK) cells have been found to possess potent antitumor responses and induce complete remissions in patients with leukemia. However, the poor infiltration of transferred NK cells is a major obstacle in developing adoptive cell immunotherapy for solid tumors. In our study, we explored the potential of using the tumor-penetrating peptide iRGD to deliver activated CIML NK cells deep into tumor tissues.

Methods

After being briefly stimulated with interleukin-12 (IL-12), IL-15, and IL-18, CIML NK cells were assessed for their phenotype and function with flow cytometry. The penetrating and killing capability of iRGD-modified CIML NK cells in tumor spheroids was revealed by confocal microscopy. The anti-tumor efficacy of th

SUBMITTER: Dong Y 

PROVIDER: S-EPMC10022190 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature