Tregs dysfunction aggravates postoperative cognitive impairment in aged mice.

ABSTRACT:

Objectives

Enhanced neuroinflammation is an important mechanism underlying perioperative neurocognitive disorders. Regulatory T cells (Tregs) play a crucial role in regulating systemic immune responses. The present study was aimed to investigate the participation of Tregs in the development of postoperative cognitive dysfunction (POCD).

Methods

Surgery-associated neurocognitive disorder was induced in 18-month-old mice subjected to internal fixation of tibial fracture. Morris water maze was used to examine mice cognitive function. Splenic Tregs were collected for RNA sequencing and flow cytometry. Levels of inflammatory factors in the circulation and hippocampus were measured by enzyme-linked immunosorbent assay. Protein presences of tight junction proteins were detected by

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC10022212 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature