Determination of potentially novel compensatory mutations in rpoc associated with rifampin resistance and rpob mutations in <i>Mycobacterium tuberculosis</i> Clinical isolates from peru.

ABSTRACT:

Background

Rifampicin (RIF) resistance in Mycobacterium tuberculosis is frequently caused by mutations in the rpoB gene. These mutations are associated with a fitness cost, which can be overcome by compensatory mutations in other genes, among which rpoC may be the most important. We analyzed 469 Peruvian M. tuberculosis clinical isolates to identify compensatory mutations in rpoC/rpoA associated with RIF resistance.

Methods

The M. tuberculosis isolates were collected and tested for RIF susceptibility and spoligotyping. Samples were sequenced and aligned to the reference genome to identify mutations. By analyzing the sequences and the metadata, we identified a list of rpoC mutations exclusively associated with RIF resistance and mutations in rpoB. We then evaluated the distri

SUBMITTER: Vargas AP 

PROVIDER: S-EPMC10022416 | biostudies-literature | 2020 Apr-Jun

REPOSITORIES: biostudies-literature