Identification of Evolutionary Trajectories Associated with Antimicrobial Resistance Using Microfluidics.

ABSTRACT: In vitro experimental evolution of pathogens to antibiotics is commonly used for the identification of clinical biomarkers associated with antibiotic resistance. Microdroplet emulsions allow exquisite control of spatial structure, species complexity, and selection microenvironments for such studies. We investigated the use of monodisperse microdroplets in experimental evolution. Using Escherichia coli adaptation to doxycycline, we examined how changes in environmental conditions such as droplet size, starting lambda value, selection strength, and incubation method affected evolutionary outcomes. We also examined the extent to which emulsions could reveal potentially new evolutionary trajectories and dynamics associated with antimicrobial resistance. Interestingly, we identifi

SUBMITTER: Seo S 

PROVIDER: S-EPMC10022597 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature