Current good manufacturing practices-compliant manufacture and measurement of biotin-labeled red blood cells.

ABSTRACT:

Background

Red blood cells (RBCs) can be labeled with N-hydroxysuccinimidobiotin (sulfo-NHS-biotin), which binds to cell surface proteins under aqueous conditions. Biotinylated RBCs can be safely infused and detected in peripheral blood samples using flow cytometry, using a fluorochrome-conjugated streptavidin (SA) detection reagent. Biotinylated RBCs have been used to track survival of transfused RBCs, and have applications in optimizing RBC storage and in understanding donor genetic, environmental and disease factors affecting RBC products.

Methods

We have developed a closed-system, current good manufacturing practices (cGMP)-compliant procedure for biotinylation of RBCs and a quantitative flow cytometric assay to estimate the dose of cell-bound biotin delivered to the pat

SUBMITTER: Donnenberg AD 

PROVIDER: S-EPMC10022648 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature