Simultaneous recoupling of chemical shift tensors of two nuclei by R-symmetry sequences.

ABSTRACT: Chemical shift tensors (CSTs) are sensitive probes of structure and dynamics. R-symmetry pulse sequences (RNCSA) can efficiently recouple CSTs of varying magnitudes in magic angle spinning (MAS) NMR experiments, for a broad range of conditions and MAS frequencies. Herein, we introduce dual-channel R-symmetry pulse sequences for simultaneously recording CSTs of two different nuclei in a single experiment (DORNE-CSA). We demonstrate the performance of DORNE-CSA sequences for simultaneous measurement of ¹³C and ¹⁵N CSTs, on a U-¹³C,¹⁵N-labeled microcrystalline l-histidine. We show that the DORNE-CSA method is robust, provides accurate CST parameters, and takes only half of the measurement time compared to a pair of RNCSA experiments otherwise requir

SUBMITTER: Porat-Dahlerbruch G 

PROVIDER: S-EPMC10023370 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature