Automated cellular stimulation with integrated pneumatic valves and fluidic capacitors.

ABSTRACT: Microfluidic technologies have proven to be a reliable tool in profiling dynamic insulin secretion from islets of Langerhans. Most of these systems rely on external pressure sources to induce flow, leading to difficulties moving to more elaborate systems. To reduce complexity, a microfluidic system was developed that used a single vacuum source at the outlet to drive fluidic transport of immunoassay reagents and stimulation solutions throughout the device. A downside to this approach is the lack of flow control over the reagents delivered to the islet chamber. To address this challenge, 4-layer pneumatic valves were integrated into the perfusion lines to automate and control the delivery of stimulants; however, it was found that as the valves closed, spikes in the flow would lead to abnorm

SUBMITTER: Adeoye DI 

PROVIDER: S-EPMC10023383 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature