TCR repertoire landscape reveals macrophage-mediated clone deletion in endotoxin tolerance.

ABSTRACT:

Background

Endotoxin tolerance (ET) is a protective mechanism in the process of sepsis, septic shock, and their sequelae including uncontrolled inflammation. Accumulating evidence has shown that peripheral T cells contribute to the induction of ET. However, what and how T-cell development contributes to ET inductions remain unclear.

Methods

Mice were intraperitoneally injected with LPS at a concentration of 5 mg/kg to establish an LPS tolerance model and were divided into two groups: a group examined 72 h after LPS injection (72-h group) and a group examined 8 days after LPS injection (8-day group). Injection of PBS was used as a control. We performed high-throughput sequencing to analyze the characteristics and changes of CD4⁺SP TCRβ CDR3 repertoires with respect

SUBMITTER: Zhao J 

PROVIDER: S-EPMC10023648 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature