Proline/serine-rich coiled-coil protein 1 inhibits macrophage inflammation and delays atherosclerotic progression by binding to Annexin A2.

ABSTRACT:

Background

Atherosclerosis (AS), the main pathological basis of life-threatening cardiovascular disease, is essentially caused by chronic macrophage inflammation. Overexpression of proline/serine-rich coiled-coil protein 1 (PSRC1) reduces macrophage inflammatory responses and delays AS development. However, the exact mechanism of PSRC1 is unclear.

Methods

Proteins interacting with PSRC1 were screened by proteomics in RAW264.7 cells, followed by RT-qPCR, immunoprecipitation and immunofluorescence to explore the specific mechanistic pathways affecting inflammation. CRISPR-Cas9 constructs for PSRC1^-/- ApoE^-/- (DKO) mice and high-fat diet-fed ApoE^-/- and DKO mice were used for AS models for in vivo experiments. Upstream transcription factors of P

SUBMITTER: Pan H 

PROVIDER: S-EPMC10023832 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature