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Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets.

ABSTRACT: Here, we present a cost-effective protocol to differentiate bovine fibro-adipogenic progenitors in a thin hydrogel sheet adherent to 96-well plates. We describe steps for the embedding and culturing of cells in alginate sheets, culture maintenance, and analysis. Compared to alternative three-dimensional (3D) models such as hydrogel-based microfibers, this approach simplifies automation while retaining efficient maturation of adipocytes. Embedded cells are still subjected to a 3D environment, but the sheets can be handled and analyzed like two-dimensional cultures.

SUBMITTER: Caponi JJ 

PROVIDER: S-EPMC10024048 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets.

Caponi Joseph J JJ   Shmushkis Jane J   Geissler Hanna H   Post Mark J MJ   Jackisch Laura L   Mall Eva M EM  

STAR protocols 20230309 2

Here, we present a cost-effective protocol to differentiate bovine fibro-adipogenic progenitors in a thin hydrogel sheet adherent to 96-well plates. We describe steps for the embedding and culturing of cells in alginate sheets, culture maintenance, and analysis. Compared to alternative three-dimensional (3D) models such as hydrogel-based microfibers, this approach simplifies automation while retaining efficient maturation of adipocytes. Embedded cells are still subjected to a 3D environment, but  ...[more]