Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets.
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ABSTRACT:
SUBMITTER: Caponi JJ
PROVIDER: S-EPMC10024048 | biostudies-literature | 2023 Mar
REPOSITORIES: biostudies-literature
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Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets.
Caponi Joseph J JJ Shmushkis Jane J Geissler Hanna H Post Mark J MJ Jackisch Laura L Mall Eva M EM
STAR protocols 20230309 2
Here, we present a cost-effective protocol to differentiate bovine fibro-adipogenic progenitors in a thin hydrogel sheet adherent to 96-well plates. We describe steps for the embedding and culturing of cells in alginate sheets, culture maintenance, and analysis. Compared to alternative three-dimensional (3D) models such as hydrogel-based microfibers, this approach simplifies automation while retaining efficient maturation of adipocytes. Embedded cells are still subjected to a 3D environment, but ...[more]
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