Establishment and application of a PCR assay for the identification of virulent and attenuated duck plague virus DNA in cotton swabs.

ABSTRACT: Duck plague is an acute, febrile, and septic infectious disease caused by duck plague virus (DPV), which causes serious harm to the duck industry in China. Ducks latently infected with DPV display a clinically healthy state, which is one of the epidemiological characteristics of duck plague. In the present study, to rapidly distinguish vaccine-immunized ducks from wild virus-infected ducks during production, a PCR assay based on the newly identified LORF5 fragment was developed to effectively and accurately identify viral DNA in cotton swab samples and was used to assess artificial infection models and clinical samples. The results showed that the established PCR method had good specificity and that only the virulent and attenuated DNA of duck plague virus was specifically amplified, as th

SUBMITTER: Wu Y 

PROVIDER: S-EPMC10024229 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature