Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats.

ABSTRACT:

Backgrounds

Kidney stone also known as urolithiasis or nephrolithiasis, is one of the oldest diseases known to medicine, however, the gene expression changes and related kidney injury remains unclear.

Methods

A calculi rat model was developed via ethylene glycol- and ammonium chloride-induction. Integrated proteomic and transcriptomic analysis was performed to characterize the distinct gene expression profiles in the kidney of calculi rat. Differential expressed genes (DEGs) were sub-clustered into distinct groups according to the consistency of transcriptome and proteome. Gene Ontology and KEGG pathway enrichment was performed to analyze the functions of each sub-group of DEGs. Immunohistochemistry was performed to validated the expression of identified proteins.

Results

SUBMITTER: Zhu W 

PROVIDER: S-EPMC10024419 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature