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Thin film notch filters as platforms for biological image processing.


ABSTRACT: Many image processing operations involve the modification of the spatial frequency content of images. Here we demonstrate object-plane spatial frequency filtering utilizing the angular sensitivity of a commercial spectral bandstop filter. This approach to all-optical image processing is shown to generate real-time pseudo-3D images of transparent biological and other samples, such as human cervical cancer cells. This work demonstrates the potential of non-local, non-interferometric approaches to image processing for uses in label-free biological cell imaging and dynamical monitoring.

SUBMITTER: Sulejman SB 

PROVIDER: S-EPMC10024701 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Thin film notch filters as platforms for biological image processing.

Sulejman Shaban B SB   Priscilla Niken N   Wesemann Lukas L   Lee Wendy S L WSL   Lou Jieqiong J   Hinde Elizabeth E   Davis Timothy J TJ   Roberts Ann A  

Scientific reports 20230318 1


Many image processing operations involve the modification of the spatial frequency content of images. Here we demonstrate object-plane spatial frequency filtering utilizing the angular sensitivity of a commercial spectral bandstop filter. This approach to all-optical image processing is shown to generate real-time pseudo-3D images of transparent biological and other samples, such as human cervical cancer cells. This work demonstrates the potential of non-local, non-interferometric approaches to  ...[more]

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