The localization of molecularly distinct microglia populations to Alzheimer's disease pathologies using QUIVER.

ABSTRACT: New histological techniques are needed to examine protein distribution in human tissues, which can reveal cell shape and disease pathology connections. Spatial proteomics has changed the study of tumor microenvironments by identifying spatial relationships of immunomodulatory cells and proteins and contributing to the discovery of new cancer immunotherapy biomarkers. However, the fast-expanding toolkit of spatial proteomic approaches has yet to be systematically applied to investigate pathological alterations in the aging human brain in health and disease states. Moreover, post-mortem human brain tissue presents distinct technical problems due to fixation procedures and autofluorescence, which limit fluorescence methodologies. This study sought to develop a multiplex immunohistochemistry a

SUBMITTER: Shahidehpour RK 

PROVIDER: S-EPMC10024857 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature